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differentiation 68  (Bio-Rad)


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    Structured Review

    Bio-Rad differentiation 68
    Differentiation 68, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 3155 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+cd68/Rat+anti+Mouse+CD68/pm42884680-117-52-57
    Average 96 stars, based on 3155 article reviews
    differentiation 68 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Temporally resolved single-cell RNA sequencing reveals protective and pathological responses during herpes simplex virus 1 CNS infection
    Article Snippet: .. The sections were incubated overnight at room temperature with the first primary antibodys monoclonal anti–HSV-1 (1/500, VP5 Abcam, clone 3B6), anti-mouse CD3ε Antibody (1/500 Biolegend, clone 145-2C11) or monoclonal rat anti mouse CD68 (1/500 Bio-Rad, clone FA-11) in 2.5% serum. .. The next day, sections were pre-blocked for 10 min in 1% serum before 2 hours of incubation with the biotinylated secondary antibody in 1% serum, (1/200, anti-mouse, pre-absorption performed in mouse tissue before adding to experimental tissue), followed by 1-hour incubation with avidin-biotin-peroxidase complex (Vectorstain, ABCkit, Vector Laboratories) in KPBS.

    Article Title: PM2.5 exposure exacerbates Alzheimer's disease pathology through lysosomal dysfunction in APP/PS1 mice.
    Article Snippet: A Leica cryostat (CM 1950, Leica, Germany) was used to cut a series of 10 μm-thick sections in the coronal plane and mounted on adhesive glass slides. .. Tissues were incubated overnight at 4 ◦ C in the following antibodies: purified anti-β-Amyloid, 1–16 Antibody (6E10, Biolegend, 803001, 1:1000), phospho-TAU (Thr231) monoclonal antibody (AT180) (Thermo Fisher Scientific, MN1040, 1:100), antiCathepsin B antibody (Abcam, ab214428, 1:250), anti-IBA1 (Wako, 019–19741, 1:1000), rat anti mouse CD68 (Bio-rad, MCA1957, 1:100), LAMP1 (Proteintech group, 65050–1-Ig, 1:200), ASC/TMS1 (D2W8U) rabbit mAb (Cell Signaling Technology, #67824, 1:1000), anti-Myelin PLP (Abcam, ab254363, 1:500) and anti-Myelin Basic (MBP) (Abcam, ab218011, 1:5000). .. On the subsequent day, glass slides were rinsed with PBST (PBS containing 0.1 % Tween-20 [Sigma, P7949]) and incubated with anti-Rabbit IgG(H+L), F(ab’)2 Fragment (Alexa Fluor® 488 Conjugate) (Cell Signaling Technology, 4412S, 1:2000) or anti-Mouse IgG (H+L), F(ab’)2 Fragment (Alexa Fluor® 594 Conjugate) (Cell Signaling Technology, 8890S, 1:2000) at room temperature for 1 h. After being washed with PBST for three times, the coverslips were mounted onto the slides using antifade mounting medium with DAPI (Beyotime Biotechnology, P0131).

    Article Title: Endocytosis of red blood cell extracellular vesicles by macrophages leads to cytoplasmic heme release and prevents foam cell formation in atherosclerosis.
    Article Snippet: .. The slides were blocked with the blocking buffer (PBS containing 2% FBS) for 40 min and then incubated with the blocking buffer containing mouse TruStain (BioLegend, Cat #: 101319) at a dilution of 1:1000 for 5 min. Antibodies against mouse HO-1 (Proteintech, Cat #: 10701- 1-AP) (1:400 dilution in PBS 2% FBS, 0.1% Triton-X100) and against mouse CD68 (Bio-Rad, Cat #: MCA1957) (1:600 dilution in PBS 2% FBS, 0.1% Triton-X100) were applied and the slides were incubated for 1 h at room temperature and then washed with the wash buffer (PBS containing 2% FBS). .. AlexaFluor 488-Anti mouse IgG secondary antibody (Jackson ImmunoResearch, Cat #: 711-545-152) and AlexaFluor 594-Anti rat IgG secondary antibody (1:500 dilution in in PBS 2% FBS, 0.1% Triton-X100) were applied for 1 h at room temperature and then washed with the wash buffer.

    Article Title: PolySialic Acid Nanoparticles Actuate Complement-Factor-H-Mediated Inhibition of the Alternative Complement Pathway: A Safer Potential Therapy for Age-Related Macular Degeneration.
    Article Snippet: Choroidal flat-mounts were labeled with Isolectin-B4 to assess the extent of neovascularization after laser CNV and against Iba-1, CD68, and complement C5b-9 to assess the choroidal microglia/macrophage inflammatory response to laser CNV. .. Eye cups were rinsed with cold IHC buffer, then incubated overnight at 4 ◦C with primary antibody cocktail in IHC buffer: rabbit antibody to mouse C5b-9 (Abcam; 1:100), goat antibody to mouse Iba-1 (Novus Biologicals, Centennial, CO, USA; 1:100), and/or rat antibody to mouse CD68 (Bio-Rad Laboratories, Hercules, CA, USA; 1:200). .. Following IHC buffer rinses, eye cups were incubated for 4 h at 4 ◦C with secondary antibody cocktail in ICC buffer: Isolectin-B4 DyLight 649 (Vector Laboratories, Newark, CA, USA; 1:200), donkey anti-rabbit Cy3 (Vector Laboratories; 1:200), donkey anti-goat Cy2 (Vector Laboratories; 1:200), and/or donkey anti-rat DyLight 405 (Vector Laboratories; 1:200).

    Article Title: PolySialic Acid Nanoparticles Actuate Complement-Factor-H-Mediated Inhibition of the Alternative Complement Pathway: A Safer Potential Therapy for Age-Related Macular Degeneration
    Article Snippet: Choroidal flat-mounts were labeled with Isolectin-B4 to assess the extent of neovascularization after laser CNV and against Iba-1, CD68, and complement C5b-9 to assess the choroidal microglia/macrophage inflammatory response to laser CNV. .. Eye cups were rinsed with cold IHC buffer, then incubated overnight at 4 °C with primary antibody cocktail in IHC buffer: rabbit antibody to mouse C5b-9 (Abcam; 1:100), goat antibody to mouse Iba-1 (Novus Biologicals, Centennial, CO, USA; 1:100), and/or rat antibody to mouse CD68 (Bio-Rad Laboratories, Hercules, CA, USA; 1:200). .. Following IHC buffer rinses, eye cups were incubated for 4 h at 4 °C with secondary antibody cocktail in ICC buffer: Isolectin-B4 DyLight 649 (Vector Laboratories, Newark, CA, USA; 1:200), donkey anti-rabbit Cy3 (Vector Laboratories; 1:200), donkey anti-goat Cy2 (Vector Laboratories; 1:200), and/or donkey anti-rat DyLight 405 (Vector Laboratories; 1:200).

    Purification:

    Article Title: PM2.5 exposure exacerbates Alzheimer's disease pathology through lysosomal dysfunction in APP/PS1 mice.
    Article Snippet: A Leica cryostat (CM 1950, Leica, Germany) was used to cut a series of 10 μm-thick sections in the coronal plane and mounted on adhesive glass slides. .. Tissues were incubated overnight at 4 ◦ C in the following antibodies: purified anti-β-Amyloid, 1–16 Antibody (6E10, Biolegend, 803001, 1:1000), phospho-TAU (Thr231) monoclonal antibody (AT180) (Thermo Fisher Scientific, MN1040, 1:100), antiCathepsin B antibody (Abcam, ab214428, 1:250), anti-IBA1 (Wako, 019–19741, 1:1000), rat anti mouse CD68 (Bio-rad, MCA1957, 1:100), LAMP1 (Proteintech group, 65050–1-Ig, 1:200), ASC/TMS1 (D2W8U) rabbit mAb (Cell Signaling Technology, #67824, 1:1000), anti-Myelin PLP (Abcam, ab254363, 1:500) and anti-Myelin Basic (MBP) (Abcam, ab218011, 1:5000). .. On the subsequent day, glass slides were rinsed with PBST (PBS containing 0.1 % Tween-20 [Sigma, P7949]) and incubated with anti-Rabbit IgG(H+L), F(ab’)2 Fragment (Alexa Fluor® 488 Conjugate) (Cell Signaling Technology, 4412S, 1:2000) or anti-Mouse IgG (H+L), F(ab’)2 Fragment (Alexa Fluor® 594 Conjugate) (Cell Signaling Technology, 8890S, 1:2000) at room temperature for 1 h. After being washed with PBST for three times, the coverslips were mounted onto the slides using antifade mounting medium with DAPI (Beyotime Biotechnology, P0131).

    Plasmid Purification:

    Article Title: PM2.5 exposure exacerbates Alzheimer's disease pathology through lysosomal dysfunction in APP/PS1 mice.
    Article Snippet: A Leica cryostat (CM 1950, Leica, Germany) was used to cut a series of 10 μm-thick sections in the coronal plane and mounted on adhesive glass slides. .. Tissues were incubated overnight at 4 ◦ C in the following antibodies: purified anti-β-Amyloid, 1–16 Antibody (6E10, Biolegend, 803001, 1:1000), phospho-TAU (Thr231) monoclonal antibody (AT180) (Thermo Fisher Scientific, MN1040, 1:100), antiCathepsin B antibody (Abcam, ab214428, 1:250), anti-IBA1 (Wako, 019–19741, 1:1000), rat anti mouse CD68 (Bio-rad, MCA1957, 1:100), LAMP1 (Proteintech group, 65050–1-Ig, 1:200), ASC/TMS1 (D2W8U) rabbit mAb (Cell Signaling Technology, #67824, 1:1000), anti-Myelin PLP (Abcam, ab254363, 1:500) and anti-Myelin Basic (MBP) (Abcam, ab218011, 1:5000). .. On the subsequent day, glass slides were rinsed with PBST (PBS containing 0.1 % Tween-20 [Sigma, P7949]) and incubated with anti-Rabbit IgG(H+L), F(ab’)2 Fragment (Alexa Fluor® 488 Conjugate) (Cell Signaling Technology, 4412S, 1:2000) or anti-Mouse IgG (H+L), F(ab’)2 Fragment (Alexa Fluor® 594 Conjugate) (Cell Signaling Technology, 8890S, 1:2000) at room temperature for 1 h. After being washed with PBST for three times, the coverslips were mounted onto the slides using antifade mounting medium with DAPI (Beyotime Biotechnology, P0131).

    Blocking Assay:

    Article Title: Endocytosis of red blood cell extracellular vesicles by macrophages leads to cytoplasmic heme release and prevents foam cell formation in atherosclerosis.
    Article Snippet: .. The slides were blocked with the blocking buffer (PBS containing 2% FBS) for 40 min and then incubated with the blocking buffer containing mouse TruStain (BioLegend, Cat #: 101319) at a dilution of 1:1000 for 5 min. Antibodies against mouse HO-1 (Proteintech, Cat #: 10701- 1-AP) (1:400 dilution in PBS 2% FBS, 0.1% Triton-X100) and against mouse CD68 (Bio-Rad, Cat #: MCA1957) (1:600 dilution in PBS 2% FBS, 0.1% Triton-X100) were applied and the slides were incubated for 1 h at room temperature and then washed with the wash buffer (PBS containing 2% FBS). .. AlexaFluor 488-Anti mouse IgG secondary antibody (Jackson ImmunoResearch, Cat #: 711-545-152) and AlexaFluor 594-Anti rat IgG secondary antibody (1:500 dilution in in PBS 2% FBS, 0.1% Triton-X100) were applied for 1 h at room temperature and then washed with the wash buffer.

    Staining:

    Article Title: Three-dimensional view of microglia-amyloid plaque interactions.
    Article Snippet: .. For other immunostainings, the protocol was the following: On the next day, sections of GFP+ mice were rinsed 3 5 min in TBS-T (TBS + 0.5% TritonX, 0.5 M, pH 7.6) and stained free floating in RT overnight in dark on a shaker table with the following primary antibodies: • Rabbit monoclonal anti-human amyloid-β 1:10000 (ref 08/20, D54D4 XPCR, Cell Signaling Technology, USA) • Rat-anti mouse CD68 (1:4000, cat. MCA1957 Bio-Rad, USA). ..

    Article Title: Three‐dimensional view of microglia—amyloid plaque interactions
    Article Snippet: .. For other immunostainings, the protocol was the following: On the next day, sections of GFP+ mice were rinsed 3 × 5 min in TBS‐T (TBS + 0.5% TritonX, 0.5 M, pH 7.6) and stained free floating in RT overnight in dark on a shaker table with the following primary antibodies: Rabbit monoclonal anti‐human amyloid‐β 1:10000 (ref 08/20, D54D4 XPCR, Cell Signaling Technology, USA) Rat‐anti mouse CD68 (1:4000, cat. MCA1957 Bio‐Rad, USA). ..

    Immunohistochemistry:

    Article Title: PolySialic Acid Nanoparticles Actuate Complement-Factor-H-Mediated Inhibition of the Alternative Complement Pathway: A Safer Potential Therapy for Age-Related Macular Degeneration.
    Article Snippet: Choroidal flat-mounts were labeled with Isolectin-B4 to assess the extent of neovascularization after laser CNV and against Iba-1, CD68, and complement C5b-9 to assess the choroidal microglia/macrophage inflammatory response to laser CNV. .. Eye cups were rinsed with cold IHC buffer, then incubated overnight at 4 ◦C with primary antibody cocktail in IHC buffer: rabbit antibody to mouse C5b-9 (Abcam; 1:100), goat antibody to mouse Iba-1 (Novus Biologicals, Centennial, CO, USA; 1:100), and/or rat antibody to mouse CD68 (Bio-Rad Laboratories, Hercules, CA, USA; 1:200). .. Following IHC buffer rinses, eye cups were incubated for 4 h at 4 ◦C with secondary antibody cocktail in ICC buffer: Isolectin-B4 DyLight 649 (Vector Laboratories, Newark, CA, USA; 1:200), donkey anti-rabbit Cy3 (Vector Laboratories; 1:200), donkey anti-goat Cy2 (Vector Laboratories; 1:200), and/or donkey anti-rat DyLight 405 (Vector Laboratories; 1:200).

    Article Title: PolySialic Acid Nanoparticles Actuate Complement-Factor-H-Mediated Inhibition of the Alternative Complement Pathway: A Safer Potential Therapy for Age-Related Macular Degeneration
    Article Snippet: Choroidal flat-mounts were labeled with Isolectin-B4 to assess the extent of neovascularization after laser CNV and against Iba-1, CD68, and complement C5b-9 to assess the choroidal microglia/macrophage inflammatory response to laser CNV. .. Eye cups were rinsed with cold IHC buffer, then incubated overnight at 4 °C with primary antibody cocktail in IHC buffer: rabbit antibody to mouse C5b-9 (Abcam; 1:100), goat antibody to mouse Iba-1 (Novus Biologicals, Centennial, CO, USA; 1:100), and/or rat antibody to mouse CD68 (Bio-Rad Laboratories, Hercules, CA, USA; 1:200). .. Following IHC buffer rinses, eye cups were incubated for 4 h at 4 °C with secondary antibody cocktail in ICC buffer: Isolectin-B4 DyLight 649 (Vector Laboratories, Newark, CA, USA; 1:200), donkey anti-rabbit Cy3 (Vector Laboratories; 1:200), donkey anti-goat Cy2 (Vector Laboratories; 1:200), and/or donkey anti-rat DyLight 405 (Vector Laboratories; 1:200).



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